Episodes
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This episode features the audio from an interview where Divya Dhasman from GenoWrite speaks with Catia Moutinho. They discuss the evolution of The Single-Cell World and explore the role of technology in advancing genomics.
Special thanks to Paul Naphtali for making this interview possible. Catch the full interview here.
Thanks to you for listen. Hit the ❤️ if you liked it and share it with your colleagues.
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In this episode, I speak with Shuwen Chen, a product manager at Takara Bio, who provides an in-depth look at the various solutions they offer for single-cell research projects. We discuss the Smart-Seq kits, explore their single-cell platforms, and dive into the latest Shasta kits for RNA and DNA. Tune in to find out if these innovative solutions can enhance your research projects.
Useful links
* Arc-well: a new method for single-cell DNA-seq analysis of formalin-fixed paraffin-embedded samples
* CUT&Tag for efficient epigenomic profiling of small samples and single cells
* Support this podcast. 👉🏻Pay us a Ko-fi
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Missing episodes?
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In this episode, I talk with Adrian Zambrano, Customer Success Manager at CYTENA. We’ll discuss instruments that can be used in single-cell projects, including upstream tools like single cell dispensation instruments and downstream solutions like automatic bead clean-up.
Discover innovative solutions from CYTENA that could enhance your research projects. Tune in to learn how these tools can make our lab work easier and more efficient.
Useful links
* Resources Cytena
* Support this podcast. 👉🏻 Pay us a Ko-fi
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BD Biosciences, known for their cytometers, also offers impressive single-cell sequencing solutions. Today’s guest is Hasi Patel, Senior Single Cell Solutions Architect, who tells me everything about this technology during this episode.
Hasi starts explaining how BD's latest high-throughput single-cell platform works, as well as all the multiomics approaches that can be done with their innovative solutions. In all of them, sample multiplexing is possible, enhancing its versatility. Hasi also shares valuable tips on planning single-cell sequencing experiments.
Tune in now to learn more about BD Biosciences' innovations in single-cell sequencing!
Useful Links:
* More information: [email protected]
* Support our podcast and subscribe to our newsletter.
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In this episode, Irene González, the International Key Account Manager at Arrays For Cell Nanodevices, SL (A4cell), discusses their microdevices known as SPAchips. She explains how these devices allow us to directly measure various characteristics in living cells, such as reactive oxygen species (ROS), calcium and pH levels.
A4Cell email: [email protected]
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Join me, Cátia Moutinho, as I break down the basics of spatial transcriptomics legal battles ⚖️ between companies in this field. I'll share my opinions 💭, propose solutions 🛠️, and raise open questions ❓ that might have you thinking. This is a message for judges, companies teams and researchers. 😉
Tune in now! 🎧
The Single-Cell World Blog, Newsletter.
Pay us a Kofi
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In this episode, Ritika Kulshreshtha, Singleron Account Manager, tells me everything about their different kits for single cell experiments. For example, a kit to study DNA and RNA in parallel, another to study glycosylation, mRNA from yeast, or nascent RNA. So, if you are interested in doing novel single cell experiments, listen to this episode now!
Useful links:
Singleron Boot Camp
Support our Podcast (2$ per month)
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Hi there 👋 The topic of this episode is VASA-seq, a single-cell transcriptomics method that we can use to study coding and non-coding RNA. Listen now to my conversation with Fredrik Salmén, the first author of the paper where VASA-seq is described. He gives me an amazing explanation about this method, besides his opinion on other topics related to single-cell and spatial experiments. You will not want to skip this one!
Other useful links:
* Pay us a Ko-fi
* SORT-seq
* Atrandi Biosciences
* Single Cell Discoveries
* Genevia Technologies
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In this podcast episode, I have the pleasure of talking with Kevin Travers, the Senior Vice President for R&D at LevitasBio.
Kevin tells me everything about their gentle and label-free technology that can be used during sample preparation for any single cell sequencing experiments, but also for other downstream applications.
🎧Tune in now and learn how levitation technology can make your experiments better.✨
Useful Links:
* Levitation Technology for Single Cell Genomics
* The LeviCell Systems for Cell Isolation and Enrichment
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Join us on this podcast episode as I explore the fascinating world of single-cell RNA sequencing (scRNA-seq) data normalization with Amartya Singh, a post-doctoral researcher at Reuters. Discover the limitations of the standard methods used in scRNA-seq data normalization and how Amartya's innovative solution is changing the game. Hear firsthand about his journey of inspiration and the ideas that led to the development of this groundbreaking approach. Don't miss out on this insightful discussion - tune in now!
Useful Links:
* Amartya Singh LinkedIn and GitHub profile
* The starting point publication
* TuBA paper
* Piccolo paper
❤️ Support our podcast: pay us a Ko-fi
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In this insightful podcast interview with Kieren Patel, the Chief Business Officer at Resolve Biosciences, we delve into the world of Molecular Cartography technology. Kieren provides details about this innovative platform, underscoring its remarkable capabilities in facilitating high molecular resolution pathology. Additionally, Kieren sheds light on forthcoming solutions set to be commercialized later this year, with a particular emphasis on their commitment to multiomics and customized approaches. Tune in to gain valuable insights into the latest advancements in molecular cartography and discover the promising future that Resolve Biosciences' offerings hold.
Useful links:
Resolve Biosciences
* website
* LinkedIn
* Twitter
* Youtube
* Datasets
Kieren Patel
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In this podcast episode, I had the privilege of talking with Melanie Masuda, the Director of Market Development at Scale Biosciences. Melanie told me all about Scale Biosciences' cutting-edge technology and gave me a full overview of all of their different kits.
Listen now and understand how the single-cell (sc) sequencing DNA methylation, the scRNAseq, and the CRISPR enrichment kits work. Maybe this is the solution for your research project!
Useful Links:
Melanie Masuda LinkedIn
Scale Biosciences(website)
* Twitter
* LinkedIn
KITS
* scDNA methylation
* scRNA
* CRISPR guide enrichment kit
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In this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family.
Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research.
In addition, we also talked about other common methods and the bigger picture of single-cell sequencing technology, such as the problems it faces and its future.
Explore the fascinating world of SMART-seq methods with Simone Picelli - a must-listen episode. Tune in now and learn about the ideal way to study RNA isoforms.
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USEFUL LINKS:
* Simone Picelli Information
* LinkedIn
* Laboratory
Publications
* SMART-Seq
* SMART-Seq 2 Original Paper
* SMART-Seq 2 Method Paper
* SMART-seq 3
* SMART-Seq 3 Express
* FLASH-seq
Protocols:
* FLASH-Seq
* FLASH-Seq UMI
* FLASH-Seq low amplification
* Bulk FLASH-Seq
Scientist.com: science as a service
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The Single-Cell World Podcast is now available in video as well, but just on Apple Podcast and in our new YouTube channel 😉
Episode 14: Single-Cell Methods, an overview, needed an update since there is a fourth method that is being used to individualize cells when using single-cell sequencing technology.
Join me and listen to it, as well as other updates concerning the methods that are at the core of the single-cell sequencing technology.
Here I leave you a gift, related with this episode.
Thank you for listening.
Kind Regards,
Cátia
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Hi there!
Today is Part 2 of the review of single-cell-related products launched in 2023. I hope I don't miss any. If I do, send me a message telling me about it. 😉
Here are some useful links:
Exploring Single-Cell Sequencing online courseCompanies that I talk during this episode (part 2):
Scale Biosciences
Scipio Bioscience
Singleron Biotechnologies
Takara Bio
10X Genomics
Free Resources
Sponsorship
Pay me a Ko-FiThanks for listening!
Cheers,
Cátia
Get full access to Single-Cell World's Substack at thesinglecellworld.substack.com/subscribe -
Hi there!
Today I review single-cell-related products launched in 2023. They are a lot! I hope I don't miss any. If I do, send me a message telling me about it. 😉
Here are some useful links:
Join the Single-Cell World NewsletterCompanies that I talk during this episode (part 1):
BD Biosciences
Ep. 18: Samples Multiplexing
BioScryb Genomics
Fluent BioSciences
Episode 31: Podcast Fluent BioSciences
Honeycomb Biotechnologies
Episode 19: Podcast Honeycomb Biotechnologies
Mission Bio
Episode 16: Podcast Mission Bio
Parse Biosciences
Episode 35: Podcast Parse Biosciences
Free Resources
Sponsorship
Pay me a Ko-FiThanks for listening!
Cheers,
Cátia
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Hello there!
The Spatial and Single-Cell Analysis Playbook was released last week. Front Line Genomics produced it. I was honored to be invited to take part in it.
In this episode, you can hear the questions that were asked (AI voice here) and my responses during this fantastic interview.
I hope you like it!
Useful Links
Free Resources
The Single-Cell World website
Sponsorship
Pay me a Ko-FiThanks for listening.
Cheers,
Cátia
Get full access to Single-Cell World's Substack at thesinglecellworld.substack.com/subscribe -
Hi there!
Would you like to know what quality control steps I think are essential before a spatial transcriptomics experiment? In my experience, these control steps can save you a lot of headaches.
Listen now, and tell me if you agree.
Useful Links
The Single-Cell World website
Sponsorship
Pay me a Ko-FiThanks for listening.
Cheers,
Cátia
Get full access to Single-Cell World's Substack at thesinglecellworld.substack.com/subscribe -
Discover the marvels of MERSCOPE®, a revolutionary platform that's reshaping the world of single-cell spatial transcriptomics. Think of it as Google Earth for genes; it allows us to directly map and quantify the spatial distribution of hundreds to tens of thousands of RNA species in individual cells. Dr. Jiang He, Vizgen’s Scientific Co-founder and Senior Director of Scientific Affairs explains everything about the MERSCOPE® technology!
Listen now to learn more about single-cell spatial technology. And if you enjoy our podcast, don't forget to share with friends and subscribe to our podcast to listen to more groundbreaking discussions! 🚀🧬
Useful links:
Vizgen website
MERSCOPE Platform
Vizgen Twitter, LinkedIn, Youtube
Dr. Jiang He Twitter and LinkedIn
Support This Podcast
Sponsorship
Pay me a Ko-FiThanks for listening.
Cheers,
Cátia
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Hi!
Would you like to get a general idea about the sample preparation standard protocols for spatial transcriptomics experiments?
Listen now 😉.
Useful links:
Youtube Videos
Frozen Tissues Preparation
FFPE Tissues Preparation
How to Cut Your Frozen Tissue Sample To Get Beautiful Sections?
OCT Blocks Prep
Support this podcast
Ko-fi
SponsorsThank you so much for listening.
Cheers,
Cátia
Get full access to Single-Cell World's Substack at thesinglecellworld.substack.com/subscribe - Show more
